Selective effects of long-term glycolytic inhibition on B cells from autoimmune prone mice

To determine the metabolic requirements of key autoimmune populations in a spontaneous lupus-prone mouse model, we carried out RNA-seq of BXSB.Cg-Cd8atm1Mak Il15tmImx Yaa (Yaa DKO) mice following glycolytic inhibition with 2-deoxyglucose (2DG). Pre-symptomatic (6-week old) mice were used as controls for symptomatic (10-week old) mice with or without long-term (4-week) treatment with 2DG. As a result, we found that 2DG-mediated glycolytic inhibition preferentially affected gene signatures of activated B cells and not T cells or other immune cell populations. Gene expression profiling by RNA-seq of spleen cells from untreated and 2DG-treated symptomatic Yaa DKO mice, and untreated pre-symptomatic Yaa DKO mice.