Single-nuclear RNA sequencing of imiquimod-applied psoriasis-like mouse skin samples

Here we performed single-nuclei RNAseq of the imiquimod-induced psoriasis-like mouse skin samples. This data will be useful supplementary data to the single-cell RNAseq data (GSE238086) of the imiquimod-treated mouse skin samples. Because large cells, such adipocytes, are often lost through enzymatic digestion in scRNA-seq. SnRNAseq can maximally capture and characterize the immune responses of these large cells. Skin biopsies were collected from adult mice (C57BL/6, 2 month, female) topically applied with imiquimod for 5 days. Nuclear suspensions were extracted from skin biopsies, and nuclei were purified by FACS sorting prior to being subjected to 10x Genomics single nuclei cDNA library prep and sequenced on an Illumina Novaseq6000 platform.