Identification of pomalidomide-dependent CRBN binding proteins
收藏NIAID Data Ecosystem2026-03-13 收录
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资源简介：
The CRL4-FH-CRBN complex was purified from 293T cells stably expressing FH-CRBN and stained with CBB following SDS-PAGE. The purified complex was incubated with various cell extracts for 24 h in the presence or absence of pomalidomide. Bound proteins were then affinity-purified using anti-HA beads and subjected to SDS PAGE and CBB staining. Gel slices were prepared and subjected to MS.
创建时间：
2022-02-02
