Identification of a motif for HLA-DR1 binding peptides using M13 display libraries.
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A M13 phage peptide display library containing 20 million independent phages was constructed using random oligonucleotides. Randomized peptide inserts were 9 amino acids in length and flanked by 4 glycines on each side. DR1-specific enrichment was performed by 4 rounds of selection (elution) and amplification (E.coli). Peptide M13P1-6 was selected at random from the DR-specific phage pool. Phage generated peptide M13P1-6 was able to out compete HA 307-319 for binding to DR1 molecules.
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IEDB创建时间:
1992-01-01



