The effect of GFP;ACT88F cross with DDRGK1 on Thoracic Muscle Development in Drosophila Melanogaster

The present study successfully established a muscle-specific GFP; ACT88F-Gal4 Drosophila experimental line through a multistage genetic crossing strategy involving balancer chromosomes and phenotypic marker selection. Sequential crosses using GFP, CyO, TM3, TM6, and TM2 balancer stocks enabled the accurate selection of progeny carrying the desired ACT88F-Gal4 driver combination. GFP fluorescence, wing morphology, and pupal size served as reliable phenotypic markers throughout the screening process. The final homozygous GFP; ACT88F-Gal4 stock was validated and used for downstream functional analyses.

Using this established genetic background, GFP; ACT88F-Gal4 flies were crossed with DDRGK1 lines to generate F1 progeny for muscle-specific functional studies. The experimental design enabled efficient assessment of gene-specific effects on locomotor and flight-associated phenotypes. Horizontal flight assay analysis demonstrated significant differences in flight performance between experimental and control groups, suggesting that DDRGK1 influences muscle function and/or neuromuscular regulation in Drosophila.

Overall, the study provides a robust framework for generating muscle-specific transgenic Drosophila models and demonstrates the utility of the ACT88F-Gal4 system for functional genetic analyses. These findings contribute to the understanding of gene regulation in muscle physiology and establish a reliable platform for future studies investigating molecular mechanisms underlying muscle-related phenotypes.