Drivers of N2O dynamics in natural and agricultural Danish streams

Nitrous oxide (N2O) emissions have been increasing rapidly over the last decades, mainly as a result of nitrogen pollution and industrialisation. Aquatic inland systems, such as rivers and streams, are found to be significant emitters of N2O. Here, we combined a year-round investigation of the physicochemical properties of ten small Danish streams (5 agriculturally impacted and 5 low-impacted in a range of varying pH) with microbial community analysis (16S rRNA gene-based) and an in-depth metagenomic analysis of two agricultural streams varying in pH value to assess the effect of pH on microbial metabolic potential.