Circular RNA expression profile in human bladder cancer cell line and human urothelial immortalized cell line

To further explore the differential expression profile of circRNA in human bladder cancer, we have employed circRNA microarray expression profiling as a discovery platform to identify potential differential expression profile of circRNA between human bladder cancer cell (UM-UC-3 cell) and urothelial immortalized cell (SV-HUC-1 cell). Results showed a large number of differentially expressed circRNAs. In this study, we screened the eight circRNAs that were most significantly downregulated in the circRNA differential expression profile of bladder cancer cells, designed primers across the splice site, and detected them using qPCR. We found that hsa_circ_0047253 (circLAMA3) was most significantly decreased in the bladder cancer cells, then its biological function and action mechanism have been further explored in this study. To explore the role of circRNA in bladder cancer, we conducted circRNA microarray analysis using human urothelial immortalized SV-HUC-1 cells as a control group and human bladder cancer UM-UC-3 cells as an experimental group.