RNA sequencing of venom glands and venom gland organoids

Both single cell and bulk RNA sequencing was performed on expanding or differentiating snake venom gland organoids (from Aspidelaps Lubricus Cowlesi and Naja Nivea), or tissue (Aspidelaps Lubricus Cowlesi). Bulk RNA sequencing from the snake venom gland, liver and pancreas was performed to construct a de novo transcriptome using Trinity. Overall design: Cells from expanding or differentiating organoids or venom gland tissue were single cell sorted in a 384 well plate before processing. For bulk RNA sequencing, RNA was extracted from organoids or whole tissue using Trizol.