pHlim source data

Changes in sub-cellular pH play a key role in metabolism, cell growth,  membrane transport, and can also be exploited to control cargo release  from therapeutic delivery systems. Most methods to measure pH rely on  intensity changes of pH sensitive fluorophores, however these  measurements are hampered by high uncertainty in the inferred pH and the  need for multiple fluorophores. To address this, we have developed a  method to accurately quantify sub-cellular pH in individual vesicles  using fluorescent lifetime imaging microscopy (pHLIM). This project  contains all the source data for the corresponding Nature Communications  article.