Time series of motor neuronal translatomes over the course of neuroinflammation

During the course of multiple sclerosis (MS), inflammatory insults drive neuro-axonal loss and disability progression. Here we leverage translating ribosome affinity purification (TRAP) to extract ribosome-bound mRNA from Chat-positive motor neurons of mice undergoing experimental autoimmune encephalomyelitis (EAE), the animal model of MS. This unique dataset allows to follow the temporal dynamics of neuronal responses to inflammation and enables the extractions of molecular targets for therapeutic intervention. mRNA of Chat+ spinal cord motor neurons derived from mice undergoing experimental autoimmune encephalomyelitis (EAE) and healthy control mice was enriched by translating ribosome affinity purification (TRAP) and sequenced by RNA-seq.