DNA interactions of transcriptional co-regulator SEUSS (SEU) during Arabidopsis flower development.

Transcriptional co-regulators are key modulators of gene expression, often functioning in complexes with transcription factors to regulate chromatin dynamics. LEUNIG_HOMOLOG (LUH) is a member of the conserved Groucho/Tup1 family of co-regulators in Arabidopsis, yet its role in flower development remains poorly understood. While LEUNIG (LUG), a homolog of LUH, has been extensively studied in floral organ identity specification, contributions of LUH appear to be both overlapping and distinct from LUG. Other co-regulators, such as SEUSS (SEU) and its homologs SEUSS-LIKE1-3 (SLK1-3), function as bridge proteins that stabilize the interactions between transcription factors and cofactors from the LUG/LUH family. Using high-throughput DNA occupancy analyses, we identify SEU target genes during flower development in Arabidopsis. Our findings contribute to understanding how LUH modulates transcriptional responses and chromatin state to fine-tune flower development in Arabidopsis. Overall design: To study the high-throughput DNA occupancy of SEU protein during flower development in Arabidopsis, we conducted chromatin immunoprecipitation followed by sequencing (ChIP-seq) experiments using inflorescences of the proSEU:SEU-GFP seu-1 plant line. Two biological replicates were prepared with the input samples as controls. We used µMACS Anti-GFP MicroBeads (Miltenyi Biotec) for the immunoprecipitation.