Transdifferentiating alveolar macrophages characterized by Placenta-expressed transcript 1 (Plet1) expression

This experiment is performed to analyse the differential expression of genome pattern on both M1 and M2 macrophages during infection in vivo. This experiment could provide us the key genes that regulate the phenotype of macrophages and the difference in expression of pro-survival and anti-apoptotic genes during influenza virus induced acute lung injury.The animlas are infected with (PR8) influenza virus and the bronchoalveolar lavage samples are collected with PBS+EDTA and stained with antibodies. later proceeded with cell sorter to sort out M1 macrophages from D7 pi and M2 macrophages from D21 pi. n = 8 animals were used, macrophages were isolated from bronchoalveolar lavage either at day 7 p.i. (n=4) or at day 21 p.i. (n=4).