Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato

Wild species are an invaluable source of new traits for crop improvement.
Over the years the tomato community bred cultivated lines that carry
introgressions from different species of the tomato tribe to facilitate
trait discovery and mapping. The next phase in such projects is to find
the genes that drive the identified phenotypes. This can be achieved by
genotyping a few thousand individuals resulting in fine-mapping that can
potentially identify the causative gene. To couple trait discovery and
fine mapping we are presenting large, recombination-rich, Backcross Inbred
Line (BIL) populations involving an unexplored accession of the wild,
green-fruited species Solanum pennellii (LA5240; the Lost Accession) with
two modern tomato inbreds: LEA, determinate, and TOP
indeterminate. The LEA and TOP BILs are in BC2F6-8 generation and include
1,400 and 500 lines respectively. The BILs were genotyped with ~5,000 SPET
markers, showing that in the euchromatic regions there was one recombinant
every 17-18 Kb while in the heterochromatin a recombinant every 600-700 Kb
(TOP and LEA respectively). To gain perspective on the topography of
recombination we compared five independent members of the self-pruning
gene family with their respective neighboring genes; based on PCR markers,
in all cases we found recombinants. Further mapping analysis of two known
morphological mutations that segregated in the BILs (Self-pruning and
Hair), showed that the maximal delimited intervals were 73 Kb and 210 Kb
respectively and included the known causative genes. The LOST_BILs provide
a solid framework to study traits derived from a tolerant
wild tomato.