Metagomic study of Epinephelus fuscoguttatus fish gut under different breeding conditions

We extracted and sequenced the total DNA out of faecal samples, orginating from Epinephelus fuscguttatus, an important Indonesian food fish species. The samples, derived under free-living and mariculture conditions, were processed by an own developed pipeline, based on blastn, to achieve taxonomical annotations for the bacterial communites and eukaryotic content and in addition,we provided a functional annotation to the sequence reads, based on Pfam Protein families. The results were analysed for their differences, regarding the two different breeding conditions, and the elucidation of eukaryotic content and bacterial communities in general.