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Figure S1 - Product Inhibition in Native-State Proteolysis

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https://figshare.com/articles/dataset/_Product_Inhibition_in_Native_State_Proteolysis_/1225557
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Deviation from first-order kinetics. (A) Proteolysis of 500 µg/ml DHFR by 80 µg/mL thermolysin was monitored by SDS-PAGE. Bands corresponding to DHFR and thermolysin were marked accordingly. The lane labeled C shows the undigested intact DHFR, which was used as the time point at t = 0. Refer to panel B for the time of each lane. (B) The change in band intensity on the gel shown in (A) was fit to a first-order rate equation. Residuals from the curve-fitting are shown below. (PDF)
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