A multi-omics investigation into the mechanisms of hyper-virulence in Mycobacterium tuberculosis

The result validated the connections between mutations, gene expression and mycobacterial pathogenicity, and identified new possible virulence-associated pathways in M. tuberculosis. We profiled the genome-wide expression pattern for hyper-virulent (H112) and low-virulent strains (H54) during exponential growth in a nutrient-rich condition (7H9 medium) and a physiologically relevant multi-stress condition (NR medium) using RNA-sequencing and label-free quantitative proteomics.