Profiling open chromatin regions in human myometrial tissues

The open chromatin regions of nonpregnant and term pregnant human myometrial tissues were assessed by ATAC-seq to gain insight on potential genome regulatory elements in vivo. patterns of accessible genome change between NP and TP myometrium, leading to altered enrichment of binding motifs for hormone and muscle regulators such as the progesterone receptor (PGR), Krüppel-like factors and MEF2A transcription factors. Three nonpregnant and three term pregnant myometrial specimens were subject to ATAC-seq analysis. Viable tissues were taken from myometrial margins at the time of hysterotomy performed for clinically indicated cesarean section. After washing each specimen briefly in phosphate buffered saline (PBS), pieces of myometrium are flash frozen. All specimens are stored at <-80°C until use. ATAC-seq was performed by the Active Motif.