DNA methylation profiles of human primed and naive pluripotent stem cells. DNA methylation profiles of human primed and naive pluripotent stem cells

We introduce a method for generating transgene-free and high-quality naive human induced pluripotent stem cells (iPSCs) using a modified Sendai virus (SeV) vector reprogramming system. This reprogramming method realizes the derivation of naive iPSCs from various somatic cells accompanied with fast and robust SeV vector removal at early passages. The established naive iPSCs have superior naive-specific differentiation ability compared with iPSCs derived from conventional methods. Overall design: Bisulphite converted DNA from the 13 samples were hybridised to the Illumina Infinium Human Methylation 450K or EPIC BeadChip Kit