Dynamics of the UV response of the foliar pathogen Fusarium mangiferae

We previously revealed developmental regulation over UV repair capacity in the soilborne pathogen Fusarium oxysporum (F. oxysporum). We demonstrated that photoreactivation assisted survival and repair were high during early stages of germination and low at later stages. In agreement, the expression of the UV specific repair genes photolyase and uvde showed opposite trends. While early on photolyase is induced and uvde is reduced, the trend is reversed later. Here, we tested the dynamic of transcription of photolyase, UV survival, repair capacity, and UV induced mutagenesis in the foliar pathogen Fusarium mangiferae. Unlike F. oxysporum, neither did we observe developmental control over photoreactivation dependent repair nor the changes in gene expression of phr1 and uvde throughout the experiment. Similarly, photo-reactivation assisted reduction in UV induced mutagenesis was similar throughout the development of F. mangiferae but fluctuated during the development of F. oxysporum. To generate hypotheses regarding the recovery of F. mangiferae after UV exposure, an RNAseq analysis was performed after irradiation at different timepoints. The most striking effect of UV on F. mangiferae was developmental-dependent induction of translation related genes. We further report a complex dynamic response that involves translation, cell cycle and lipid biology related genes. Germinating condia of Fusarium mangiferae strain MRC7560 at either 8 or 14 h post innoculation. 8 h post innoculation spores were irradiated with either 50 or 200J/m2 and allowed to recover for additional 30 m. 14 h post innoculation spores were irradiated with either 50 or 200J/m2 and allowed to recover for additional 0, 30, or 60 m. 8 h or 14 h untreated populations were used as a control. Each treatment includes three biological replicates.