DataSheet_1_Exploring the antibacterial and dermatitis-mitigating properties of chicken egg white-synthesized zinc oxide nano whiskers.docx

IntroductionZinc oxide nanoparticles (ZnO-NPs) have garnered considerable interest in biomedical research primarily owing to their prospective therapeutic implications in combatting pathogenic diseases and microbial infections. The primary objective of this study was to examine the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) using chicken egg white (albumin) as a bio-template. Furthermore, this study aimed to explore the potential biomedical applications of ZnO NWs in the context of infectious diseases.MethodsThe NWs synthesized through biological processes were observed using electron microscopy, which allowed for detailed examination of their characteristics. The results of these investigations indicated that the NWs exhibited a size distribution ranging from approximately 10 to 100 nm. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) mapping analyses successfully corroborated the size, dimensions, and presence of biological constituents during their formation. In this study, XTT assay and confocal imaging were employed to provide evidence of the efficacy of ZnO-NWs in the eradication of bacterial biofilms. The target bacterial strains were Staphylococcus aureus and Escherichia coli. Furthermore, we sought to address pertinent concerns regarding the biocompatibility of the ZnO-NWs. This was achieved through comprehensive evaluation of the absence of cytotoxicity in normal HEK-293T and erythrocytes.ResultsThe findings of this investigation unequivocally confirmed the biocompatibility of the ZnO-NWs. The biosynthesized ZnO-NWs demonstrated a noteworthy capacity to mitigate the dermatitis-induced consequences induced by Staphylococcus aureus in murine models after a therapeutic intervention lasting for one week.DiscussionThis study presents a comprehensive examination of the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) derived from chicken egg whites. These findings highlight the considerable potential of biosynthesized ZnO-NWs as a viable option for the development of therapeutic agents targeting infectious diseases. The antibacterial efficacy of ZnO-NWs against both susceptible and antibiotic-resistant bacterial strains, as well as their ability to eradicate biofilms, suggests their promising role in combating infectious diseases. Furthermore, the confirmed biocompatibility of ZnO-NWs opens avenues for their safe use in biomedical applications. Overall, this research underscores the therapeutic promise of ZnO-NWs and their potential significance in future biomedical advancements.

引言氧化锌纳米颗粒（ZnO-NPs）在生物医学研究领域备受关注，其主要原因在于其在对抗病原性疾病和微生物感染方面的潜在治疗意义。本研究的主要目标是探讨利用鸡蛋白（白蛋白）作为生物模板的生物合成氧化锌纳米线（ZnO-NWs）的过程。此外，本研究旨在探讨ZnO-NWs在感染性疾病背景下的潜在生物医学应用。方法通过电子显微镜观察通过生物过程合成的纳米线，这允许对它们的特性进行详细检查。这些调查的结果表明，纳米线的大小分布范围约为10至100纳米。傅里叶变换红外光谱（FTIR）和扫描电子显微镜-能量色散X射线光谱（SEM-EDX）映射分析成功证实了纳米线的大小、尺寸以及在其形成过程中的生物成分的存在。在本研究中，XTT试验和共聚焦成像被用于提供ZnO-NWs在根除细菌生物膜方面的有效性的证据。目标细菌菌株为金黄色葡萄球菌和大肠杆菌。此外，我们试图解决关于ZnO-NWs生物相容性的相关担忧。这通过全面评估正常HEK-293T细胞和红细胞中不存在细胞毒性来实现。结果这项研究的发现明确证实了ZnO-NWs的生物相容性。生物合成的ZnO-NWs显示出显著的减轻由金黄色葡萄球菌引起的皮炎后果的能力，这种能力在为期一周的治疗干预后在小鼠模型中得以体现。讨论本研究全面考察了源自鸡蛋白的氧化锌纳米线（ZnO-NWs）的生物合成。这些发现突出了生物合成ZnO-NWs作为针对感染性疾病治疗剂可行选择的巨大潜力。ZnO-NWs对易感菌和耐抗生素菌菌株的抗菌功效，以及它们根除生物膜的能力，预示着它们在对抗感染性疾病中的潜在作用。此外，ZnO-NWs的生物相容性得到证实，为它们在生物医学应用中的安全使用开辟了途径。总体而言，这项研究强调了ZnO-NWs的治疗前景及其在未来的生物医学进步中的潜在重要性。