Number of productive primers generated for tested loci.

Simulated amplicons were made using generated primers. Non-productive primers did not amplify sequences capable of being distinguished with a restriction digest. Problematic primers amplify sequences capable of being distinguished, but the reaction would likely not amplify DNA due to primer defects such as 3’ mismatches or gaps in alignment to provided sequence. Productive primers are expected to successfully amplify DNA capable of being distinguished by a restriction digestion.