Integrated transcriptome analysis of radioresistant cells revealed genes and pathways predictive of tumor response to radiotherapy and chemotherapy in breast cancer

Radiotherapy, in addition to surgery and chemotherapy, remains the core of the current clinical management of breast cancer. Although radiotherapy is effective in most patients, some of them will develop recurrent disease because of radioresistant tumor cell. MCF-7RR and MDA-MB-231RR cell lines were established from their parental MCF-7 and MDA-MB-231 cells parental cells were irradiated with a linear accelerator Cells received 15 sequential fractions of 2 Gy week, allowing irradiated cell populations a period of recovery between exposures. Non-irradiated controls were handled identically to the irradiated cells without radiation exposure. All of the experiments were performed within 4–10 passages after the final irradiation