Mitochondrial respiration rates, ROS production and OXPHOS content.

Data are presented as mean±SEM and significance was determined by Student’s T-test analysis between WT and C5L2KO on same diet, where *p<0.05, #p = 0.08. State 3 (ADP-stimulated respiration) was induced upon the addition of ADP (450 uM), state 4 (leak respiration) was chemically attained with the addition of the ATP-synthase inhibitor oligomycin (1 µg/mL) and the maximally uncoupled state (maximum oxygen flux) was achieved by titrating the chemical uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).