Induction of spontaneous human neocentromere formation and long-term maturation (NativeCA ChIP-Seq)

We report a chromosome engineering system for human neocentromere formation and characterize the first experimentally generated human neocentromere. Neocentromere formation promotes local H3K9me3 eviction and cohesin and RNA polymerase II recruitment. Long-term culture results in gradual maturation of the inner centromere. CENP-A occupancy in survivor clone after endogenous centromere deletion containing a neocentromere to detect the exact genomic position of this neocentromere formation event.