CoMtb alters the immune landscape following Mtb infection [C3H scRNAseq]

To gain further insights into lesion development and effects of CoMtb on immune responses during infection. we performed single-cell RNA sequencing (scRNAseq) and flow cytometry analysis of lungs from Mtb-infected animals with and without CoMtb immediately pre-infection and at 10. 17 and 34 days after CD infection. We found that CoMtb induces rapid recruitment and activation of T cells. macrophage activation. and diminished late neutrophil responses. Overall design: Single-cell RNA sequencing (scRNAseq) and flow cytometry analysis of lungs from Mtb-infected C3H mice with and without CoMtb immediately pre-infection and at 10. 17 and 34 days after CD infection. Variables tested including comparing timepoints to each other. and CoMtb vs non-CoMtb