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Comparing shotgun vs. amplicon sequencing analysis of environmental samples of invertebrates.. Mitogenomics vs. metabarcoding of environmental samples.

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB23036
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资源简介:
New applications of DNA and RNA sequencing are expanding the field of biodiversity discovery and ecological monitoring, yet questions remain regarding precision and efficiency. We used a set of documented mock communities comprising 13 species of freshwater macroinvertebrates of estimated individual biomass, to compare the detection efficiency of COI metabarcoding (3 different amplicons) and shotgun mito-metagenome sequencing. Additionally, we used individual COI barcoding and de novo mitogenome sequencing, to provide reference sequences for OTU assignment and mito-metagenome assembly respectively.
创建时间:
2018-04-02
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