Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer [scCRISPR]

The gene regulatory network (GRN) underlying CTL differentiation and whether Tex responses can be functionally reinvigorated are incompletely understood. Here, we systematically mapped causal GRNs via single-cell CRISPR (scCRISPR) screens in vivo and discovered checkpoints for CTL differentiation. We used scCRISPR to identify the TF regulatory network of transcriptional heterogeneity of OT-I Tcells at day 7 post adoptive transfer into B16-OVA tumors