RNA-seq profiling of six fetal tissues at day 63 of gestation from a reciprocal backcross of German Landrace and Piétrain

Utilizing a reciprocal backcross design: F1 (German Landrace × Pietrain) sows × GL or Pi sires (Ponsuksili et.al. 2025; doi:https://doi.org/10.1098/rsob.240285), we profiled six fetal tissues (liver, brain, muscle, kidney, ovary, and testis) at day 63 of gestation. Our study aimed to: (i) characterize transcriptomic differences both between backcross groups (F1×GL and F1×Pi) across tissue types; (ii) map tissue-specific ASE landscapes within each backcross group; and (iii) identification of differential ASE (dASE) genes by comparing allele-specific expression between reciprocal backcrosses within each tissue. All together, we provide insights into paternal genetics potentials of fetal programming for animal breeding.