Xanthocillin resistant mutants in Acinetobacter baumannii

Acinetobacter baumanii reference strain wild-type DSM30007 sensitive to Xanthocillin was exposed to growing concentrations of this antibiotic in 11 rounds of culturing, resulting in selected mutants exhibiting resistance. Shotgun genomic sequencing were obtained from wild type strain, from resistant isolates at different stages of the 11 rounds of increasing selection, from strains with the same amount of generations without antibiotic selection representing accumulated random genetic drift. The objective was to find the gene mutations responsible for the selected resistance.