RNA-seq analysis for IRF4-driven zebrafish tumors after JQ1 treatment

RNA-seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes after JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish. After the development of lymphoma phenotype, animals were treated with JQ1 or control DMSO for 1 or 3 days. Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression. Total RNAs were harvested from each sample and then applied for RNA-seq analysis.