Transcriptomic responses to oil, a pathogen, or both in spleen tissues from juvenile red snapper (Lutjanus campechanus)

The objective of this study was to determine how transcriptomic responses in the spleen are altered in juvenile red snapper exposed to oil, a known fish pathogen (Vibrio anguillarum), or both. Overall design: Red snapper juveniles were exposed to oil, a pathogen (Vibrio anguillarum) or both. Oil exposure was for 8 days and pathogen exposure was for 1 hour, 24 hours before takedown. Oil solutions used in exposures were derived from high energy water accommodated fractions (HEWAF) prepared using Macondo well oil from the Deepwater Horizon oil spill following established procedures. At the end of the experiments, spleens were taken from organisms, RNA was extracted and sequenced.