Fighting Microbes with Microbes: Multimodal Dataset on Microbioclaim and Biofilm-Mediated Corrosion Control

This dataset contains the experimental, analytical, and bioinformatic data supporting the study "Living commensal biofilms for corrosion prevention." It provides a comprehensive, multimodal evaluation of microbiome engineering as a sustainable strategy to mitigate microbiologically influenced corrosion (MIC) on copper surfaces. Specifically, the data demonstrates the efficacy of the commensal bacterium Citrobacter sp. strain MICI21 in outcompeting the highly corrosive sulfate-reducing bacterium (SRB) Oleidesulfovibrio alaskensis G20. The repository encompasses the four-part ecological framework of microbioclaim, including competitive surface occupation, biological passivation, and commensal antagonism. Data contained in this repository includes: Electrochemical Data: Open Circuit Potential (OCP), Electrochemical Impedance Spectroscopy (EIS) Nyquist/Bode plots, and potentiodynamic polarization curves demonstrating 3- to 15-fold higher corrosion resistance. Surface & Structural Characterization: Scanning Electron Microscopy (SEM) micrographs, Confocal Laser Scanning Microscopy (CLSM) surface coverage profiles, nanoindentation metrics (stiffness/elasticity), and pit-depth quantifications. Physicochemical Analysis: Gravimetric (weight-loss) assay results, Extracellular Polymeric Substance (EPS) characterization, Energy-Dispersive Spectroscopy (EDS), X-ray Diffraction (XRD), and reactor H₂S quantification. Genomic & Bioinformatic Context: 16S rRNA analysis data and outputs from comparative genomic analysis (STRING-DB protein-protein interaction networks) of publicly available Citrobacter strains highlighting putative Type VI secretion system (T6SS) antagonism features. (Note: KBase Narratives #182783 and #182791 are hosted externally). Keywords: Microbiologically Influenced Corrosion (MIC), Biofilm Engineering, Living Coatings, Citrobacter, Sulfate-Reducing Bacteria (SRB), Electrochemical Impedance Spectroscopy (EIS), Biological Passivation, Type VI Secretion System (T6SS), Copper Corrosion