RNA Sequencing analysis on Tumor associated macrophages (TAMs) Isolated form Lewis Lung Carcinoma (LLC) tumors. RNA Sequencing analysis on Tumor associated macrophages (TAMs) Isolated form Lewis Lung Carcinoma (LLC) tumors

The goal of this study is to compare transcriptome profiling of miR-21 null TAMs vs. WT macrophages isolated from LLC tumors Overall design: LLC cells were subcutaneously implanted in 8 weeks old WT or miR-21 null mice. After 14 days tumors were isolated and collagenase digested then macrophages were FACS sorted as (CD45+, CD11b+, MHCII+, F4/80) cells and RNA was extracted for subsequent RNA-seq analysis.