Role of Lsd1 in HOTAIR function

HOTAIR is a scafold long non-coding RNA tethering PRC2 and Lsd1/REST/coREST complexes to gene promoters to repress transcription of genes involved in epithelial to mesenchymal transition (EMT). To decipher a role of the Lsd1 in HOTAIR function we generated epithelial cell lines expressing full length and truncated versions of HOTAIR missing first 5'-300 (Epi-HOT?P) and last 3'-500 bp (Epi-HOT?L) interacting with HOTAIR partners. These cell lines were further used to determine Lsd1 binding sites using chromatin immunoprecipitation approach. Overall design: For all cell lines, crosslinked chromatin was purified from two independent biological duplicate cell cultures. For each replicate, an aliquot was kept as Input while the rest was used for Chromatin Immunoprecipitation using the antibody against Lsd1.