Replication Data for: The effect of injectable platelet-rich fibrin and platelet-rich fibrin in regenerative endodontics: a comparative in vitro study

Objective: To explore the feasibility of injectable platelet-rich fibrin (i-PRF) in regenerative endodontics by comparing the effect of i-PRF and platelet-rich fibrin (PRF) on biological behaviors and angiogenesis of human stem cells from apical papilla (SCAPs).
Methodology: i-PRF and PRF were obtained from venous blood at two different centrifugation ways, followed by hematoxylin-eosin (HE) staining and scanning electron microscope (SEM). Enzyme-linked immunosorbent assay (ELISA) was conducted to quantify the growth factors. SCAPs were cultured with different concentrations of i-PRF extract (i-PRFe) and PRF extract (PRFe), and the optimal concentrations were selected by the Cell Counting Kit-8 (CCK-8) assay. Then the cell proliferation and migration potentials of SCAPs were observed using the CCK-8 assay and the Transwell assay. The mineralization ability was detected by alizarin red staining (ARS) and angiogenesis capacity was detected by tube formation assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was performed to evaluate the genes expression related to mineralization and angiogenesis. Data were subjected to statistical analysis.
Results: i-PRF and PRF presented a similar three-dimensional fibrin structure while i-PRF released a higher concentration of growth factors than PRF (P < .05). 1/4× i-PRFe and 1/4× PRFe were selected as the optimal concentrations. The cell proliferation rate of i-PRFe group was higher than that of PRFe group (P < .05), while no statistical difference was observed in cell migration between them (P > .05). More importantly, our results showed that i-PRFe had a stronger effect on SCAPs than PRFe in facilitating mineralization and angiogenesis with the consistent result of RT-qPCR (P < .05).
Conclusion: This work revealed that i-PRF released a higher concentration of growth factors and was superior to PRF in promoting proliferation, mineralization and angiogenesis of SCAPs, suggesting that i-PRF could be a promising biological scaffold applied in pulp regeneration.