Table S1 - Analysis of Tp53 Codon 72 Polymorphisms, Tp53 Mutations, and HPV Infection in Cutaneous Squamous Cell Carcinomas

Primers for Tp53 analysis. The Tp53 mRNA template was transcribed into cDNA with MLV reverse transcriptase, using the listed cDNA amplification primers (1) to generate a 1142 bp product. Nested PCR reactions were carried out to amplify an 873 bp fragment from exon 4 to exon 8 using the nested PCR amplification primers (2). Both strands of the amplified cDNA were then sequenced using the specific sequencing primers (3). (DOCX)