Data from: The extracellular domains of IgG1 and T cell-derived IL-4/IL-13 are critical for the polyclonal memory IgE response in vivo

IgE-mediated activation of mast cells and basophils contributes to
protective immunity against helminths but also causes allergic responses.
The development and persistence of IgE responses are poorly understood,
which is in part due to the low number of IgE-producing cells. Here, we
used next generation sequencing to uncover a striking overlap between the
IgE and IgG1 repertoires in helminth-infected or OVA/alum-immunized
wild-type BALB/c mice. The memory IgE response after secondary infection
induced a strong increase of IgE+ plasma cells in spleen and lymph nodes.
In contrast, germinal center B cells did not increase during secondary
infection. Unexpectedly, the memory IgE response was lost in mice where
the extracellular part of IgG1 had been replaced with IgE sequences.
Adoptive transfer studies revealed that IgG1+ B cells were required and
sufficient to constitute the memory IgE response in recipient mice. T
cell-derived IL-4/IL-13 was required for the memory IgE response but not
for expansion of B cells from memory mice. Together, our results reveal a
close relationship between the IgE and IgG1 repertoires in vivo and
demonstrate that the memory IgE response is mainly conserved at the level
of memory IgG1+ B cells. Therefore, targeting the generation and survival
of allergen-specific IgG1+ B cells could lead to development of new
therapeutic strategies to treat chronic allergic disorders.