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High efficiency of gene deletion via RNP transformation was tested by FGSG_04274 for verification.

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Figshare2022-06-03 更新2026-04-28 收录
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Deletion mutants were identified by the dark red color and abnormal shapes of the colonies. *50 bp indicates that GEN is amplified by 70 bp primers, which contain 50 bp arms homologous to sequences upstream or downstream from FGSG_04274.

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2022-06-03
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