High efficiency of gene deletion via RNP transformation was tested by FGSG_04274 for verification.
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Deletion mutants were identified by the dark red color and abnormal shapes of the colonies. *50 bp indicates that GEN is amplified by 70 bp primers, which contain 50 bp arms homologous to sequences upstream or downstream from FGSG_04274.
创建时间:
2022-06-03



