Vibrio natriegens, a Promising Chassis from Strain Property to Protein Expression: A Review

Protein expression is an important aspect of synthetic
biology,
where host selection critically impacts the production efficiency. Vibrio natriegens (V. natriegens) has emerged as a promising prokaryotic host due to its exceptionally
rapid growth rate and broad substrate metabolic spectrum. Benefiting
from the advances in annotation on microbial genomes and metabolic
pathways, as well as the development of molecular biology tools, continuous
progress has been made in bioproduction using V. natriegens. In this Perspective, we provide a systematic introduction of the
basic biological properties of V. natriegens and present the engineered V. natriegens chassis which are currently used for protein expression. We summarize
the latest synthetic biology components and genetic tools specifically
designed for the modification of V. natriegens. Especially, we focus on the most recent study and application achievements
of V. natriegens in in vivo and in vitro protein expression, which has received
limited attention in previous reviews. This review aims to present
researchers with a comprehensive overview of using V. natriegens for protein expression, highlight its
advantages and application prospects, and present some challenges
and future perspectives in this field, so as to promote wider research
in the synthetic biology field.