Hypoxia disrupts sex-specific physiology and gene expression leading to decreased fitness in the estuarine sea anemone Nematostella vectensis

Coastal seawater hypoxia is increasing in temperate estuaries under global climate change, yet it is unknown how low oxygen conditions affect most estuarine species. We found that hypoxia has increased since the 1990s in an estuary hosting the sea anemone Nematostella vectensis (Jacques Cousteau National Estuarine Research Reserve, New Jersey, USA). Adult N. vectensis bred from anemones collected in this estuary exposed to three consecutive nights of hypoxia (dissolved oxygen = 0.5–1.5 mg L−1 for ~12 h night−1) during gametogenesis displayed decreased aerobic respiration rates and biomass, indicating metabolic disruption. Physiological declines were correlated with changes in the expression of genes related to oxygen-dependent metabolic processes, many of which are targets of hypoxia-inducible factor 1α (HIF1α), demonstrating the activity of this transcription factor for the first time in this early-diverging metazoan. The upregulation of genes involved in the u..., , , # Data from: Hypoxia disrupts sex-specific physiology and gene expression leading to decreased fitness in the estuarine sea anemone Nematostella vectensis

Dataset includes files for all data associated with experiments.

## Data and file structure

### File 1: Adult\_combined\_dry\_weight\_data.csv

This file is for data pertaining to the dry weight of adult sea anemones collected at several time points during the experiment.

These files have headers, which include:
-Treatment = dissolved oxygen treatment to which anemones were exposed, either normoxia or hypoxia
-Day = day of experiment on which data were collected
-Anemone_dry_weight_g = the dry weight of an individual anemone measured in grams

### File 2: Adult\_fecundity\_data.csv

This file is for data pertaining to the reproductive metrics quantified in anemones upon spawning induction. This file contains NA values as a result of combining data from female and male animals into a single data sheet. NA values indicate sex-specif...,