Table3_Development and Validation of a Novel Mitochondrion and Ferroptosis-Related Long Non-Coding RNA Prognostic Signature in Hepatocellular Carcinoma.XLSX

Mitochondrion and ferroptosis are related to tumorigenesis and tumor progression of hepatocellular carcinoma (HCC). Therefore, this study focused on exploring the participation of lncRNAs in mitochondrial dysfunction and ferroptosis using public datasets from The Cancer Genome Atlas (TCGA) database. We identified the mitochondrion- and ferroptosis-related lncRNAs by Pearson’s analysis and lasso-Cox regression. Moreover, real-time quantitative reverse transcription PCR (RT-qPCR) was utilized to further confirm the abnormal expression of these lncRNAs. Based on eight lncRNAs, the MF-related lncRNA prognostic signature (LPS) with outstanding stratification ability and prognostic prediction capability was constructed. In addition, functional enrichment analysis and immune cell infiltration analysis were performed to explore the possible functions of lncRNAs and their impact on the tumor microenvironment. The pathways related to G2M checkpoint and MYC were activated, and the infiltration ratio of regulatory T cells and M0 and M2 macrophages was higher in the high-risk group. In conclusion, these lncRNAs may affect mitochondria functions, ferroptosis, and immune cell infiltration in HCC through specific pathways, which may provide valuable insight into the progression and therapies of HCC.

线粒体与铁死亡与肝细胞癌（HCC）的肿瘤发生和进展密切相关。因此，本研究聚焦于利用来自癌症基因组图谱（TCGA）数据库的公共数据集，探讨长链非编码RNA（lncRNA）在线粒体功能障碍和铁死亡中的参与作用。通过皮尔逊分析和lasso-Cox回归，我们识别了与线粒体和铁死亡相关的lncRNA。此外，实时定量逆转录聚合酶链反应（RT-qPCR）被用于进一步验证这些lncRNA的异常表达。基于八个lncRNA，构建了具有卓越分层能力和预后预测能力的MF相关lncRNA预后标志（LPS）。此外，通过功能富集分析和免疫细胞浸润分析，探究了lncRNA的可能功能和它们对肿瘤微环境的影响。与G2M检查点和MYC相关的通路被激活，高风险组中调节性T细胞和M0、M2巨噬细胞的浸润比例更高。总之，这些lncRNA可能通过特定途径影响HCC中的线粒体功能、铁死亡和免疫细胞浸润，这或许能为HCC的进展和治疗提供宝贵的见解。