Multiomics Analysis Reveals the Involvement of JsLHY in Controlling Aroma Production in Jasmine Flowers

The Jasminum sambac flower is famous for its rich
fragrance. However, our knowledge of the regulatory network for its
aroma formation remains largely unknown and therefore needs further
study. To this end, an integrated analysis of the volatilomics and
transcriptomics of jasmine flowers at different flowering stages was
performed. The results revealed many candidate transcription factors
(TFs) may be involved in regulating the aroma formation of jasmine,
among which the MYB-related TF LATE ELONGATED HYPOCOTYL (JsLHY) was identified as a hub gene. Using the
DNA affinity purification sequencing method, dual-luciferase reporter,
and yeast one-hybrid assays, we demonstrate that JsLHY can bind the
gene promoter regions of six aroma-related structural genes (JsBEAT1, JsTPS34, JsCNL6, JsBPBT, JsAAAT5, and Js4CL7) and directly promote their expression. In addition,
suppressing JsLHY expression decreased both the expression
of JsLHY-bound genes and the content of related VOCs. The present
study reveals how JsLHY participates in jasmine aroma formation.