Bacterial rapid adaptation to alkaline environments. Bacterial rapid adaptation to alkaline environments

To investigate the rapid adaptation mechanism of Bacillus thuringiensis in an alkaline environment, we have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between normal condition and alkaline condition. Overall design: Bt HD73 strain was cultured in LB at 30 °C and 220 rpm. NaOH was added at a concentration of 28 mM to the cultures when the OD600 reached 0.7. After 10 minutes of incubation, total RNA was extracted