Comprehensive Proteomic Analysis of Exosomes Derived from Human Bone Marrow-, Adipose Tissue-, and Human Umbilical Cord- Mesenchymal Stem Cells
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Mesenchymal stem cells (MSCs)-derived exosomes (exo) have shown comprehensive application prospects over the years. Despite similar functions, exomes from different origins present heterogeneous characteristics and components; however, there are no relevant proteomic analyses. In this study, we isolated exosomes from MSCs, derived from different tissues, by ultracentrifugation. A total of 1014 proteins were detected using a label-free method and analyzed with bioinformatics tools. The results revealed their shared function in the extracellular matrix receptor. Bone marrow-MSCs-derived exosomes showed superior regeneration ability. Likewise, adipose tissue-MSCs-derived exosomes played a significant role in immune regulation. Whereas, umbilical cord-MSCs-derived exosomes were more prominent in tissue damage repair.
多年来，间充质干细胞（Mesenchymal stem cells, MSCs）衍生的外泌体（exosomes, exo）已展现出全面的应用前景。尽管功能相似，但不同来源的外泌体存在异质性特征与组分差异，然而目前尚无针对此类外泌体的相关蛋白质组学分析。本研究通过超速离心法分离得到不同组织来源的间充质干细胞外泌体，采用无标记（label-free）检测方法共检出1014种蛋白质，并利用生物信息学工具进行分析。结果显示，此类外泌体在细胞外基质受体通路中发挥共有功能。其中，骨髓源间充质干细胞外泌体表现出更优异的再生能力；脂肪组织源间充质干细胞外泌体则在免疫调控中扮演重要角色；而脐带源间充质干细胞外泌体在组织损伤修复方面更为突出。
