Analysis of the transcriptome response to low-nitrogen in Populus ussuriensis

For the RNA-seq experiments, the upper three internodes were excised from 4-week-old plantlets and transferred via micro-cutting to normal nitrogen WPM(NN), which contains 10 mM NO3? and 5 mM NH4?. After three weeks of rooting, the rooted plantlets were subjected to low-nitrogen (LN) treatment by transferring them to modified WPM containing 0.05 mM NO3? and 0.025 mM NH4?. Root samples were collected at 0, 6, 12, 24, 48, 96, and 336 hours after LN, each process is repeated three times. Overall design: RNA-seq of Populus ussuriensis roots