RNA-Seq of zebrafish embryos (96hpf) treated with different concentrations of Methimazole against untreated control groups

In the present study transcriptome analysis was employed to investigate the early molecular responses to Methimazole (CAS 60-56-0), a thyroid peroxidase inhibitor. Zebrafish embryos were exposed to methimazole according to OECD guidelines (OECD test No. 236). At the end of exposure time (96 hours), RNA was extracted from 10 embryos using a Macherey & Nagel RNA/protein extraction kit. The obtained RNA extracts were sequenced using Illumina NovaSeq 6000 system (Illumina Inc., San Diego, USA) and the obtained sequences went through bioinformatic analysis pipeline to Identify and count the detected gene sequences followed by differential gene expression analysis. Finally, potential thyroid disruption specific biomarker candidates were selected based on the differential expression patterns and the biological functions investigation of the detected differentially expressed genes (DEGs).