Table 1 in New psychropotid species (Echinodermata, Holothuroidea, Elasipodida) of the Western Pacific with phylogenetic analyses
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Table 1. PCR amplification procedures.
PrimerSequence 5′→ 3'PCR procedureCOI-efATAATGATAGGAGGRTTTGGPre denaturation: 95 °C for 3 minCOI-erGCTCGTGTRTCTACRTCCAT40 cycles:Denaturation: 95 °C for 40 sAnnealing: 45 °C for 40 sExtension: 72 °C for 50 s16S-arLCGCCGTTTATCAAAAACATPre denaturation:95 °C for 3 min16S-brHCCGGTCTGAACTCAGATCACG35 cycles:Denaturation: 95 °C for 40 sAnnealing: 50 °C for 40 sExtension: 68 °C for 50 s
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2025-04-04
