Supplementary Figure 7
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Supplementary Figure 7. NMR titration of unlabeled TPR2A to 15N-labelled psyn129. a. The concentration of psyn129 was kept constant at 100 µM (estimated by Lowry assay), while the concentrations of TPR2A varied from 0 µM to 200 µM (estimated by Lowry assay) in increments of 25 µM. The KD value was determined based on the titration data of D119, D121, and E137, the three residues that displayed the largest chemical shift changes upon the addition of TPR2A. Protein concentrations determined by amino acid analysis were used to in fitting the KD value.
创建时间:
2022-09-02



