Genome Resources of Fusarium oxysporum f. sp. vasinfectum race 1, 4, 5 and 8 that causes wilt diseases in cotton

Fungi in the genus Fusarium are hyphomycetes that are widely distributed in soils and that are often associated with plants and plant diseases. Fusarium oxysporum f.sp. vasinfectum (FOV) was first recognized in 1892, in sandy acid soils in Alabama (USA) and causing a disease called Fusarium wilt of cotton. FOV includes several pathogenic races and has been separated into 4 distinct phylogenetic subgroups based in DNA sequences of a few housekeeping genes. We have developed WGS for isolates of FOV races 1, 4, 5 and 8 using Nanopore sequencing (MinION) and Illumina (Mi-Seq) technology and assembled the contiguous contigs for individual isolates of race 1 (52 Mb), race 4 (68 Mb), race 5 (68 Mb) and race 8 (55 Mb), each FOV race draft contiguous contigs polished with high quality Illumina reads over 25 million and over 99% estimated genome completeness. These genomes will provide a necessary resource for comparative genomics to identify genes or genome features that are involved in pathogenicity, and in different regulatory and diagnostics applications.

隶属于Fusarium属的真菌为菌丝状子囊菌，广泛分布于土壤中，常与植物及其病害相关联。Fusarium oxysporum f.sp. vasinfectum（FOV）首次于1892年在美国阿拉巴马州的沙质酸性土壤中被识别，并引起被称为棉花萎蔫病的病害。FOV包含多个病原型，根据少数管家基因的DNA序列被划分为4个不同的系统发育亚组。我们利用Nanopore测序（MinION）和Illumina（Mi-Seq）技术，针对FOV的1号、4号、5号和8号病原型进行了全基因组测序（WGS），并组装了各个分离株的单个连续序列。其中，1号病原型的连续序列长度为52 Mb，4号和5号病原型的连续序列长度均为68 Mb，8号病原型的连续序列长度为55 Mb。每个FOV病原型的连续序列均经过超过2500万次高质量Illumina读数的精修，估计基因组完整性超过99%。这些基因组将为比较基因组学提供必要的资源，以识别参与致病性、不同调控和诊断应用的基因或基因组特征。