Quantification Of E-Cadherin Expression In H1299 Nsd3 Knockdown Cells
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<strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong> <strong>Exp023 Objective: </strong>Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting.
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Zenodo创建时间：
2018-05-23
